By Barbara J. Bain FRACP FRCPath, Imelda Bates MB BS MD MA FRCPath, Mike A Laffan DM FRCP FRCPath, S. Mitchell Lewis BSc MD FRCPath DCP FIBMS
Well-known all over the world because the typical reference paintings, Dacie & Lewis functional Haematology is a must-have reference for any haematology laboratory. It covers the entire innovations utilized in the research of sufferers with blood issues, together with the most recent applied sciences in addition to the attempted and precise handbook equipment of size. It discusses the rules of every try out, attainable explanations of blunders, the reason for selecting one process over one other and the translation, importance and scientific relevance of those findings. each one bankruptcy conforms to a template, offering easy access to key details
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Additional info for Dacie and Lewis Practical Haematology: Expert Consult: Online and Print
0 ¼ millimolar coefficient extinction; d ¼ layer thickness in cm; and 1000 ¼ conversion of mg to g. 27 Practical Haematology When assigning a value to a haemoglobin solution that may be used as a reference preparation, it is necessary first to calibrate the spectrometer. This requires checking wavelength with a holmium oxide filter, absorbance with a set of calibrated neutral density filters and stray light with a neutral density filter at 220 nm (National Physical Laboratory, Teddington, UK). Matched optical or quartz glass cuvettes with a transmission difference of <1% at 200 nm should be used.
It gives results that are at least 2% lower than those given by the other methods, probably because a small proportion of inert pigment is always present. The iron content of haemoglobin can be estimated accurately,1 but again the method is impractical for routine purposes. Estimations based on iron content are generally taken as authentic, but iron bound to inactive pigment is included. 2 MEASUREMENT OF HAEMOGLOBIN CONCENTRATION USING A SPECTROMETER (SPECTROPHOTOMETER) OR PHOTOELECTRIC COLORIMETER Two methods are in common use: (1) haemiglobincyanide (HiCN; cyanmethaemoglobin) method and (2) oxyhaemoglobin (HbO2) method.
3. PLATELET COUNT The method for manual counting of platelets using a counting chamber is described on p. 610. If an RBC by a semiautomated counter is available, it is possible to obtain an approximation of the platelet count by counting the proportion of platelets to red cells in a thin part of a film made from an EDTA-anticoagulated blood sample, using the Â100 oil-immersion objective and, if possible, eyepieces provided with an adjustable diaphragm, as for a reticulocyte count. 39 This maturation is thought to take 2–3 days, of which about 24 h are spent in the circulation.