By Sukriti Nag
Univ. of Toronto, Canada. textual content offers the main points of chosen morphologic, permeability, shipping, in vitro, and molecular strategies for BBB experiences. every one half is preceded by means of a evaluation emphasizing the benefits and pitfalls of specific options. Written for researchers in BBB. DNLM: Blood--Brain Barrier--physiology.
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Extra resources for Blood'Brain Barrier: Biology and Research Protocols
Rev. Cytol. 61, 167–228. 219. Vorbrodt, A. W. (1993) Morphological evidence of the functional polarization of brain microvascular endothelium. In The Blood-Brain Barrier. Cellular and Molecular Biology. (Pardridge, W. ), Raven, New York, pp. 137–164. 220. Brightman, M. , and Kaya, M. (2000) Permeable endothelium and the interstitial space of brain. Cell. Mol. Neurobiol. 20, 111–130. 32 Nag 221. Sims, D. E. (2000) Diversity within pericytes. Clin. Exp. Pharmacol. Physiol. 27, 842–846. 222. Thomas, W.
7. Morphometry Measurements of various parameters of pial and intracerebral cortical vessels such as diameter, cross sectional area of the media and intima and density of cerebral microvessels are available in the literature (5–7). In addition, measurements are also available of lengths of tight junctions, density of fenestrations and organelles in cerebral endothelium such as mitochondria, and endothelial vesicles (8–10). Details on how to perform these measurements are beyond the scope of this chapter because it depends to a certain extent on the type of image analyser being used.
An early study of frozen yeast cells suggested that the fracture planes follow the surface of membranes (13). It was then demonstrated that the fracture plane runs through the middle of the membrane, cleaving both lipid layers of the unit membrane since in a vitrified matrix the hydrophobic membrane centre is fragile enough to be easily split From: Methods in Molecular Medicine, vol. 89: The Blood–Brain Barrier: Biology and Research Protocols Edited by: S. , Totowa, NJ 51 52 Wolburg, Liebner, and Lippoldt Fig.