By Jonathan Y. Richmond, Robert W. McKinney
Describes the mixtures of normal & certain microbiological practices, security equip., & amenities that are steered for paintings with a number of infectious brokers in quite a few labs. This revision takes under consideration such concerns as international main issue approximately rising & reemerging infectious ailments; elevated curiosity in prion illnesses with the id of BSE in England; the prevalence of numerous lab-associated infections; elevated drawback concerning the nationwide & intl. move of infectious microorganisms; & becoming issues approximately bioterrorism, & the elevated protection wishes of our microbiological labs.
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Extra resources for Biosafety in Microbiological and Biomedical Laboratories
Policies for the safe handling of sharps are instituted. 6. All procedures are performed carefully to minimize the creation of splashes or aerosols. 7. Work surfaces are decontaminated on completion of work or at the end of the day and after any spill or splash 21 Laboratory Biosafety Level Criteria – Biosafety Level 2 8. 9. of viable material with disinfectants that are effective against the agents of concern. All cultures, stocks, and other regulated wastes are decontaminated before disposal by an approved decontamination method such as autoclaving.
7. If there is a central vacuum system , it does not serve areas outside the cabinet room . In-line HEPA filters are placed as near as practicable to each use point or se rvice coc k. Filte rs ar e inst alled t o per mit in-place decontamination and replacement. Other 44 Laboratory Biosafety Level Criteria – Biosafety Level 4 liquid and gas services to the cabinet room are protected by devices that prevent backflow. 8. If water fountains are provided, they are automatically or foot-operated and are located in the facility corridors outside the laboratory.
Reu sab le clot hing is dec onta min ated befo re be ing lau nde red. C lothin g is changed when overtly contaminated. 2. Gloves must be worn when handling infectious materials, infected animals, and when handling contaminated equipm ent. Frequent changing of gloves accompanied by hand washing is recommended. Disposable gloves are not reused . 3. 4. , are condu cted in a C lass II or C lass III biologic al safety cabinet (see Appendix A). 5. , centrifuge safety cups or sealed rotors) are used. 6.