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Extra resources for Animal Cell Culture: A Practical Approach 3rd Edition
IAN FRESHNEY When the cells from the outgrowth are trypsinized and reseeded into a fresh vessel they become a secondary culture, and the culture is now technically a cell line. 05% pure) or collagenase (200-2000 U/ml, crude) and the cell suspension allowed to settle on to, adhere, and spread out on the substrate (3, 4). This type of culture gives a higher yield of cells though it can be more selective, as only certain cells will survive dissociation. In practice, many successful primary cultures are generated using enzymes such as collagenase to reduce the tissue, particularly epithelium, to small clusters of cells which are then allowed to attach and grow out.
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