Acute Leukemias IX: Basic Research, Experimental Approaches by T. Haferlach, C. Schoch, W. Hiddemann (auth.), Prof. Dr.

By T. Haferlach, C. Schoch, W. Hiddemann (auth.), Prof. Dr. med. Wolfgang Hiddemann, Prof. Dr. Dr. med. phil. Torsten Haferlach, Dr. med. Michael Unterhalt, Prof. Dr. med. Thomas Büchner, Prof. Dr. med. Jörg Ritter (eds.)

Show description

By T. Haferlach, C. Schoch, W. Hiddemann (auth.), Prof. Dr. med. Wolfgang Hiddemann, Prof. Dr. Dr. med. phil. Torsten Haferlach, Dr. med. Michael Unterhalt, Prof. Dr. med. Thomas Büchner, Prof. Dr. med. Jörg Ritter (eds.)

Show description

Read Online or Download Acute Leukemias IX: Basic Research, Experimental Approaches and Novel Therapies PDF

Similar hematology books

Diagnostic & Therapeutic Advances in Hematologic Malignancies (Cancer Treatment and Research)

There isn't any box of medication during which advances in remedy have been so heavily associated with a greater figuring out of molecular drugs than within the sector of hematologic malignancies. Diagnostic and healing Advances in Hematologic Malignancies will offer practitioners and educational clinicians with a greater figuring out of the regularly evolving innovations during this region.

Endothelial Cells in Health and Disease

This reference serves because the first resource to assemble present information from endothelial mobilephone biologists in numerous disciplines to summarize contemporary growth within the field-providing a whole realizing of the endothelium in wellbeing and fitness and sickness and demonstrating its strength as a healing objective.

Phenotypic and Genotypic Diagnosis of Malignancies: An Immunohistochemical and Molecular Approach

Conscientiously designed as a simple and quickly reference, this computer guide is for operating pathologists who have to ensure the actual kind of melanoma they're facing in a sufferer. To this finish, the ebook comprises many huge tables of data to permit a quick research of effects, delivering all of the suitable details to diagnose the total diversity of other tumors in people.

Lecture notes. / Haematology

Haematology Lecture Notes is an obtainable and concise best-selling research advisor, outlining the body structure, pathology and therapy of the most typical blood issues. commonly revised and up to date to mirror the massive advances within the figuring out of the molecular biology and pathogenesis of haematological issues, it presents the middle wisdom required by means of all scientific scholars and junior medical professionals.

Extra info for Acute Leukemias IX: Basic Research, Experimental Approaches and Novel Therapies

Example text

24 hours later medium was replaced and antibiotics were added (6001200 fig/ml G41S, 150-300 Ilg/ml Zeocin). Dead cells were removed by ficoll gradient 2-6 days later. Individual stably transfected clones were obtained by limiting dilution on 96-well plates. Clones were maintained in 400-600 fig/ml G41S and 100-150 Ilg/ml Zeocin. Analysis of clones 152 clones derived from 12 independent transfections were treated with 1 11M hormone (either Muristerone A or Ponasterone A, Invitrogen, Groningen, NL) and screened for inducible AMLl/ETO protein expression by Western blotting using AMLl- and ETOspecific antibodies (kindly provided by Scott W.

25 Results and Discussion CD34+-progenitor cells were isolated from fresh cord blood at a purity of at least 95%. Virus titer reached up to 1Q6/ml supernatant. The overall transduction efficiency, determined as the ration of colony forming unit (CFU) in the MC assay with or without G418, was 10%. First results indicate that AMLI/ETO transduced cells in liquid culture exhibit a less differentiated morphology with a reduced percentage of granulated cells with prominent vacuolization (Fig. 1, 2). 3).

Yamamoto, C. , Downing, J. , Selsted, M. , and Hiebert, S. W. (1998). The t( 8;21) fusion product, AML-l/ETO, associates with C/EBPa, inhibits C/EBPa-dependent transcription, and blocks granuloctyic differentiation, Mol Cell BioI 18, 322-333. Disturbed Differentiation of AMl1lETO Transduced Human (034+ Progenitors J. BASECKEt, CH. BAUM 2 , O. PFAHLERT l , G. BRITTINGERt, B. WORMANN3, and F. GRIESINGER l Introduction Material and Methods The translocation t( 8;21) (q22;q22), AMLl/ETO, one of the most frequent genetic aberrations in acute myelogenous leukemia is detectable in 40% of AML FAB M2.

Download PDF sample

Rated 4.96 of 5 – based on 21 votes